Andrew is a freelance marine biologist in North Carolina focused on population and conservation genetics in hydrothermal vent communities.



David is a graduate student in Florida. He studies the ecology and conservation of sharks.




Amy is a graduate student in North Carolina studying local ecological knowledge within small scale fisheries.



Chuck is a graduate student in North Carolina focusing on apex predators and how they interact with fisheries.




Lyndell is a graduate student in North Carolina, studying the feeding ecology of cownose rays.




Iris is a graduate student in Washington studying habitat use and feeding habits of juvenile Pacific salmon and herring in Puget Sound.



Michael is a graduate student in Maryland investigating the visual systems of mantis shrimp.



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What does a day of discarded plastic from a molecular lab look like?

Plastic consumables from a molecular lab. Photo by Andrew David Thaler.

To complement my post earlier today on the need for best practice guidelines to minimize plastic waste in a conservation genetics lab, I asked my labmates to save all of their consumables from a day of molecular benchwork. The above picture (Sharpie for scale) is the result. All told, we produced about 1/2 kilogram of plastic (it’s hard to get a precise weight, since many of the tubes still contain liquid). With only 3 people working a the bench, this was a relatively light day.

Establishing Best Practices to Minimize Waste in a Conservation Genetics Lab

I am, among other things, a conservation geneticist. What that means is that I use the tools of molecular ecology and population genetics to make observations about species and populations in at-risk ecosystems, assess the status of anthropogenically disturbed populations, and generate data that has direct applications to conservation and management issues. Essentially, the only difference between what I do and what a population geneticist or molecular ecologist does is the motivation—I select systems to work in that have a high conservation priority.

This motivation leads to a constant intellectual conflict at the bench. The tools of molecular ecology—PCR, gene sequencing, and, more frequently, high-throughput sequencing—are waste intensive. In order to avoid cross-contamination and practice precise, clean, technique, we use thousands of tiny plastic consumables every day. These come in the form of pipette tips, sterile packaging material, micro-centrifuge tubes, and numerous other plastic widgets. Often, because of the biohazard potential, these consumable cannot be recycled.

So we have a problem. As a conservation geneticist, we need these tools to produce the data necessary to make wise conservation and management decisions. As a sustainability minded individual, I find the massive daily accumulation of plastic waste inexcusable. Do we just accept this waste as the cost of conservation genetics? I believe that the answer is no. I think we can and should develop best practices to minimize the amount of plastic waste produced by a molecular lab while maintaining good, sterile technique. I would like to propose four guidelines, based off the principles of Reduce, Reuse, and Recycle, for minimizing waste in a conservation genetics lab.

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