WhySharksMatter’s first PCR!

As promised, here are the results of my first ever PCR. Here is some background:

I am going to be running some population genetics on sandbar shark DNA with the intention of comparing subpopulations from South Carolina with those from Virginia.

I am in the very early stages- seeing which primers work for PCR. Four primers each were tested- called A, B, C and D- on three shark DNA samples and a negative control. Ignore the samples on the bottom, they are from another student’s project. The four samples in the upper right are my negative controls.

The PCR was run yesterday (my first PCR), and I ran the gel today (my first gel).

It seems to me that Primer A is successfully copying my DNA during PCR, while B, C, and D are not.

March 25, 2010 • 3:35 pm